Manitoba Workplace First Aid Requirements The first aid requirements for businesses in Manitoba are based on the number of employees working on a given shift.One Manitoba Kit is required for every 25 workers to a maximum of 4 kits. A first aid room is required for 100+ workers. For the most current regulations, refer to Part 5: Section 5.1 – 5.15 (pg. 68 of 355) and Schedule B & C (pg. 77 of 355) of the Manitoba Workplace Health and Safety Regulations. 1 Employee 1 Manitoba Personal First Aid Kit 1-24 Employees 1 Manitoba First Aid Kit 25-50 Employees 2 …